The gold-bearing pyrite samples in different production ranges and generations from the Xishimen quartz-vein gold deposit were investigated by micro-Raman spectroscopic measurement. The Raman stretching vibration frequency of two texture type was observed in gold-bearing pyrite: One was the stretching vibration frequency of Fe—［S2］2- within 338～343 cm-1; Another was the stretching vibration frequency of Au+—［S2］2- within 374～379 cm-1. Different production ranges and generations of pyrite had different Raman spectra characteristics: All the peaks shift of Au-bearing pyrite had large intensity, big changes and towards low-frequency due to the stretching vibration of Au+—［S2］2-; All the peaks shift of Au-poor pyrite had low intensity, small changes and towards high-frequency due to the stretching vibration of Fe—［S2］2-; The study showed that the main reason of the changes of the Raman spectral was gold-bearing, so the Raman spectral characteristics of gold-bearing pyrites can be used to evaluate gold-bearing properties and geological environment of gold deposits.