The objectives in the present paper were to study the relationship between the polysaccharide content and the spectrum, aimed at finding a non destructive testing method for the measurement of polysaccharide content. Materials in this study were from tissue culture seedlings under different treatments and domesticated plants in different growth stages. In the study, 36 samples were used to build estimation models and another 11 as test samples to examine the models. The relationship between the spectrum and polysaccharide content was investigated by partial least squares regression (PLSR) and factor analysis. The results show that (1) there was stronger correlation between derivative reflectance and polysaccharide content, and the sensitive wavebands mainly concentrated in the visible region. (2) The PLSR model has a high precision, while its prediction accuracy is lower. The models of factor analysis have higher prediction accuracy, the RPD of the model based on spectral reflectance is 2.269 and that of derivative reflectance is 2.305.