A multi-focus image fusion method based on region of image and multi-resolution of wavelet transform is presented. First, images involved in fusion are decomposed into pyramids. Then the approximation of each layer of the pyramids is segmented into regions to produce a Labeling image by using the method "clustering region pixels" . In the Labeling image, each region is corresponding to an object and the same value is given for the pixel of the region. Different region has different value. Secondly, a binary decision map of each layer of the pyramids is produced by means of Labeling image and the rule of selecting the pixel that the region energy is big. Each layer of the pyramid has a binary decision map. Finally, the fused image is reconstructed. The method's performance was evaluated by using error of mean square root. The experiment results show that it has good effective. The performance is better than the performance of the fusion method based on window and the fusion method proposed in the literature [3]