• Acta Optica Sinica
  • Vol. 41, Issue 6, 0617001 (2021)
Hui Hua1、2、3, Gaofang Yin1、3、*, Nanjing Zhao1、3、**, Tingting Gan1、3, Min Chen1、2、3, Lu Wang1、2、3, Peilong Qi1、2、3, Xiang Wang1、2、3, Xiaoling Zhang4, Li Fang1、3, Ruifang Yang1、3, Ming Dong1、2、3, Zhihao Chen1、2、3, Renqing Jia1、2、3, Zhichao Ding1、2、3, and Jianguo Liu1、3
Author Affiliations
  • 1Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, China
  • 2University of Science and Technology of China, Hefei, Anhui 230026, China
  • 3Anhui Provincial Key Laboratory of Optical Monitoring Technology for Environment, Hefei, Anhui 230031, China
  • 4Institutes of Physical Science and Information Technology, Anhui University, Hefei, Anhui 230601, China
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    DOI: 10.3788/AOS202141.0617001 Cite this Article Set citation alerts
    Hui Hua, Gaofang Yin, Nanjing Zhao, Tingting Gan, Min Chen, Lu Wang, Peilong Qi, Xiang Wang, Xiaoling Zhang, Li Fang, Ruifang Yang, Ming Dong, Zhihao Chen, Renqing Jia, Zhichao Ding, Jianguo Liu. Study on Selecting Characterization Parameters of Viable Algae Cells Number in Ballast Water Based on Chlorophyll Fluorescence Kinetics[J]. Acta Optica Sinica, 2021, 41(6): 0617001 Copy Citation Text show less
    Change trend of photosynthetic fluorescence parameters with the increase of dead cells number
    Fig. 1. Change trend of photosynthetic fluorescence parameters with the increase of dead cells number
    Change trend of photosynthetic fluorescence parameters with the increase of CDOM concentration
    Fig. 2. Change trend of photosynthetic fluorescence parameters with the increase of CDOM concentration
    Changes of photosynthetic fluorescence parameters with viable algae cells number in different diluents. (a) Fm; (b) F0; (c) Fv; (d) [RCII]
    Fig. 3. Changes of photosynthetic fluorescence parameters with viable algae cells number in different diluents. (a) Fm; (b) F0; (c) Fv; (d) [RCII]
    T-test results between the same type photosynthetic fluorescence parameters with seawater as a diluent and those with other fluorescence interference solution as a diluent
    Fig. 4. T-test results between the same type photosynthetic fluorescence parameters with seawater as a diluent and those with other fluorescence interference solution as a diluent
    Quantitative relationship of photosynthetic fluorescence parameters to viable algae cells number. (a) Fv; (b) F0; (c) Fm; (d) [RCII]
    Fig. 5. Quantitative relationship of photosynthetic fluorescence parameters to viable algae cells number. (a) Fv; (b) F0; (c) Fm; (d) [RCII]
    Trend diagram of photosynthetic fluorescence parameters and viable algae cells number under DCMU stress
    Fig. 6. Trend diagram of photosynthetic fluorescence parameters and viable algae cells number under DCMU stress
    ParameterMeasurement or derivationPhysiological significance
    FmAt the asymptote of a single turnover (ST) pulseMaximum yields of chlorophyll a fluorescencemeasured after dark adaptation
    F0Extrapolation to t=0 from a single turnover (ST) pulseMinimum yields of chlorophyll a fluorescencemeasured after dark adaptation
    FvFm-F0Variable fluorescence
    σPSIIFluorescence kinetic curve (the rate of the fluorescencerise is proportional to the functional absorption crosssection of PSII (σPSII))Functional absorption cross section for PSII
    [RCII]F0/σPSIIEffective photosynthetic reaction center concentration
    Table 1. Photosynthetic fluorescence parameters acquired by FRRf
    FluorescenceparameterCorrelationcoefficient(diluted seawater)Correlationcoefficient(mixture)
    Fm0.989**-0.692
    F00.995**-0.914**
    Fv0.955*0.955**
    [RCII]0.997*-0.845
    Note:** represents p<0.01,* represents p<0.05.
    Table 2. Correlation analysis between the photosynthetic fluorescence parameters and viable algae cells number in diluted seawater and mixture
    ParameterCorrelation coefficient
    Fm-0.997**
    F0-0.999**
    Fv0.986**
    [RCII]-0.888*
    Note:** represents p<0.01,* represents p<0.05.
    Table 3. Correlation analysis between photosynthetic fluorescence parameters and viable algae cells number under the stress of DCMU
    Hui Hua, Gaofang Yin, Nanjing Zhao, Tingting Gan, Min Chen, Lu Wang, Peilong Qi, Xiang Wang, Xiaoling Zhang, Li Fang, Ruifang Yang, Ming Dong, Zhihao Chen, Renqing Jia, Zhichao Ding, Jianguo Liu. Study on Selecting Characterization Parameters of Viable Algae Cells Number in Ballast Water Based on Chlorophyll Fluorescence Kinetics[J]. Acta Optica Sinica, 2021, 41(6): 0617001
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