• Advanced Imaging
  • Vol. 1, Issue 1, 011004 (2024)
Julien Guilbert1, Awoke Negash1, Simon Labouesse2, Sylvain Gigan1..., Anne Sentenac2 and Hilton B. de Aguiar1,*|Show fewer author(s)
Author Affiliations
  • 1Laboratoire Kastler Brossel, ENS-Université PSL, CNRS, Sorbonne Université, Collège de France, Paris, France
  • 2Aix Marseille Univ, CNRS, Centrale Marseille, Institut Fresnel, Marseille, France
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    DOI: 10.3788/AI.2024.10004 Cite this Article Set citation alerts
    Julien Guilbert, Awoke Negash, Simon Labouesse, Sylvain Gigan, Anne Sentenac, Hilton B. de Aguiar, "Label-free super-resolution stimulated Raman scattering imaging of biomedical specimens," Adv. Imaging 1, 011004 (2024) Copy Citation Text show less

    Abstract

    Far-field super-resolution microscopy has unraveled the molecular machinery of biological systems that tolerate fluorescence labeling. Conversely, stimulated Raman scattering (SRS) microscopy provides chemically selective high-speed imaging in a label-free manner by exploiting the intrinsic vibrational properties of specimens. Even though there were various proposals for enabling far-field super-resolution Raman microscopy, the demonstration of a technique compatible with imaging opaque biological specimens has been so far elusive. Here, we demonstrate a single-pixel-based scheme, combined with robust structured illumination, that enables super-resolution in SRS microscopy. The methodology is straightforward to implement and provides label-free super-resolution imaging of thick specimens, therefore paving the way for probing complex biological systems when exogenous labeling is challenging.
    ΔIS{χ(3)}IPIS,

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    ΔIS(x,y){χ(3)(x,y)}IP(x,y)IS(xx,yy)dxdy({χ(3)}IP)IS.

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    Julien Guilbert, Awoke Negash, Simon Labouesse, Sylvain Gigan, Anne Sentenac, Hilton B. de Aguiar, "Label-free super-resolution stimulated Raman scattering imaging of biomedical specimens," Adv. Imaging 1, 011004 (2024)
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