To understanding the energy transfer from carotenoids molecular to reaction centre in PSII, PSII particle complex purified from spinach was investigated with ultrafast fluorescence spectroscopy in the case of selective excited carotenoid molecular with 473 nm, 481nm and 507 nm at 77 K. The data were analyzed by fluorescence decay time-fitting, and were compared with data of core complex got at the same condition. Three groups of time components about energy transfer of carotenoids were obtained: 16.6ps, 130~183 ps, 217~249 ps. The shortest lifetime component of 16.6 ps was the process of energy transfer from Car 473 molecular to Chl 639 in LHCII. The 130~183 ps were lifetime associated with the process of β-car molecular transfer the energy to RC by a series of intermediates and 217~249 ps reflected the process of Car_ 481 in LHCII transfer it′s energy to RC.