The principle of knife-edge thermometry is introduced. A chromium beam which is jetted from high temperature atomic crucible is collimated by one-dimensional laser Doppler cooling on self-designed system of chromium atom lithography. The laser wavelength in vacuum is 425.5 nm, corresponding to 7S3→7P04 transition of 52Cr. The setup of knife-edge thermometry is designed and built to quantify the effect of the chromium beam collimation by laser cooling. The obvious cooling phenomenon is observed with power of 45 mW, spot size of 1 mm×20 mm and detuning of -2.5 MHz. Then, based on it, the knife-edge measurement is carried out. The results show that the full-width at half-maximum (FWHM) of chromium beam angular distribution is (0.616±0.007) mrad and the transverse temperature is (418±10) μK by laser cooling. The results provide a basis for optimizing the chromium beam collimation by laser cooling.