TiC enhanced coating has excellent properties, such as high hardness, wear resistance, oxidation resistance, etc., and it is widely used on the surface of tools and mold. Laser cladding is an ideal method of preparing TiC enhanced coating, but the usually used mechanical mixing powder method is even more difficult to mix the powder even, which directly affects the generation of TiC particles. A new method of sol-gel is used to prepare even TiO2-graphite mixed powder, and the TiC enhanced coating is made by TiO2-C in-situ synthesis through diode laser irradiation on 45# steel substrate. The phases on the coating surface, microstructure and hardness of the enhanced layer are tested by X-ray diffraction, scanning electron microscopy, micro-hardness tester, respectively. The influences of laser power, scanning speed, gas flow on TiC distribution and microstructure of enhanced coating are investigated. The results show that the shape of generated TiC particles in enhanced coating is polygonal or irregular. On the premise that the cladding layer can be formed and TiC particles can be generated, the energy density is smaller and the gas flowing speed is bigger, more TiC particles is generated in the coating. The maximum hardness of enhanced coating reaches 1151 HV0.2 when laser processing specific energy is 250 W·s/mm2 and gas flow rate is 30 L/min.