Holes with diameter from 0.3～1 mm need to be processed in micro-sprinkler irrigation plastic pipeline for agriculture. This requirement cannot be satified by traditional way of using mechanical punching. Laser drilling in such pipeline not merely brings some advantages such as good suitability in material, high efficiency in production and free burr on porthole but also improves product quality and increases variety of product. The CW fiber laser and pulsed fiber laser have been employed on drilling in micro-sprinkler irrigation plastic pipeline with ingredient of PE(Polyethylene) in the experiment. The dependence between laser power and hole diameter and the dependence between fluence and hole diameter have been achieved. Finally, holes for required diameter have been made in the pipeline by using CW fiber laser and pulsed fiber laser.