The enhanced fluorescence effect of Rh6G molecules deposited on physically polished copper surface was studied. The result shows that the formation of oxidation film on the metal surface plays an important role in the fluorescence emission. The quench and enhanced surface fluorescence effect from the physically polished rough copper surface with/without oxide layer were experimentally observed. The formative oxidation film was helpful to decrease the efficiency of the noradiative energy transfer from the fluorophore to the metal surface, and enhanced fluorescence intensity will be obtained with the properly controlling of the spacer layer between the molecules and metal surface. The experimental observation was analyzed with the local field of surface enhanced fluorescence.