The techniques of portable energy-dispersive X-ray fluorescence analysis (PXRF), proton induced X-ray emission (PIXE), X-ray diffraction (XRD) and laser Raman spectroscopy (LRS) were employed to analyze the chemical composition， structure and mineral constitution of 12 samples. The results indicated that the chemical compositions determined by PXRF and PIXE are well comparable and most samples are constituted by almost pure jadeite with low concentration of secondary elements. One sample contains a little omphacite and one sample is composed only by omphacite. Raman characteristic peaks of jadeite occurred at 201, 372, 698, 985 and 1 037 cm-1, while those of omphacite located at 680 and 1 017 cm-1. By using laser Raman spectroscopy for testing the fissures of the samples， wax in 3 samples and epoxy resin in one sample were found. Raman characteristic peaks of wax located at 2 846 and 2 880 cm-1 and those of epoxy resin occurred at 2 924 and 3 065 cm-1. The application of nondestructive techniques in jadeite jade broadens the range of samples for future study and provides technical support for jadeite jade’s further study, identification and classification.