Optical guidance means is used to guide the landing boat to enter the well-dock at night effectively and quickly. According to the rectangular light field shaping method with small divergence angles, the light emitting diode (LED) guiding source with 2.3°×1.15° full width at half maximum is designed. An optical alignment method based on guiding light source colors or flicker frequency is put forward. Based on the guidance system strategy, five LED guiding sources with different colors or flicker frequencies are manufactured and the system prototype is built. Experimental results show that the prototype can effectively realize the alignment function of the landing boat optical guidance system.