The targeted imaging of quantum dot (QD) conjugated cyclo (Arg-Gly-Asp-D-Phe-Lys ) peptides [c(RGDfK), QD-RGD] for laryngeal cancer blood vessel in vivo is studied. QD is conjugated with c(RGDfK) peptides by the reaction of carboxyl and amino groups. The spectra stabilities of QD-RGD in RMPI1640 and mouse serum are measured by fluorescence spectrophotometer. The targeting of QD-RGD to αvβ3 on Hep-2 and MCF-7 cells is studied by fluorescent microscope. Finally, the targeting of QD-RGD to laryngeal cancer vascular in dorsal skin fold window chamber by tail intravenous injection is investigated. The result shows that the spectra stability of QD-RGD in RPMI1640 does not obviously change in 4 hours. The fluorescence intensity of QD-RGD in mouse serum in 24 hours only decreases by 20%.The result of cells fluorescence imaging shows that QD-RGD can specifically bind to integrin αvβ3 on cells. The result of vascular imaging shows QD-RGD gathers in cancer blood vessel after injecting for 2 hours, and QD-RGD is removed from cancer blood vessel after 24 hours. The study demonstrates that QD-RGD can be used to targeted image cancer blood vessel in vivo, which offers a reference for studying targeting diagnosis and targeting therapy of laryngocarcinoma in vivo.