Ho3+/Yb3+ doped germanate glasses adapting for K+-Na+ ion-exchanged waveguide were fabricated. Upconversion fluorescence has been measured by absolute spectral measurement system under 975 nm diode laser excitation. Net absorption and emission photon distributions and quantum yields were derived from absolute spectral power distributions. Experimental and calculation results revealed that the effective diffusion coefficient of K+-Na+ thermal ion-exchange is 0.068 μm2/min, when Ho3+/Yb3+ doped germanate glasses are immersed in KNO3 molten salt at 370℃ for 4 hours. Ho3+ in germanate glasses emits 548 nm green and 660 nm red fluorescence, and the red one plays dominant role. The absolute spectral powers and the net emission photon numbers of the red upconversion emission are 28.03 μW and 9.26×1013 cps, when pump power density is adjusted to 1 227 W/cm2. The fluorescence quantum yields of 548 nm green and 660 nm red emissions are 0.17×10－5 and 2.41×10－5, respectively, and the total quantum yield in the visible region can reach to be 2.61×10－5 simultaneously. The slope of logarithmic curve of net emission photon number versus excitation power density indicate that the red and green upconversion emissions in Ho3+/Yb3+ doped germanate glasses are both due to two-photon excitation processes. Absolute characterization for the upconversion fluorescence from Ho3+ in waveguide-typed germanate glasses have been achieved, which provides reliable reference in developing rare earth optoelectronic functional materials.