To achieve high-efficiency delivery of bio-ink， a novel cell delivery device based on air-assisted atomization was developed，and its atomization characteristics and their effect on cell viability were studied. According to the Rosin-Rammler droplet size distribution law， the uniformity index was quantitatively evaluated. Multi-frame image superposition and local threshold binarization were used to extract the boundary of atomization from a video， so that the atomization angle could be analyzed. By combining the BM3D denoising and binarization algorithm to accurately locate the droplet positions， we could calculate the velocity of the droplets. The atomization height and the liquid flow rate are positively correlated to the diameter of the droplets. The pressure of the assistant air has a noticeable effect on the droplet uniformity when this pressure is higher than 60 kPa. By controlling the liquid flow rate and spray height， the spray area can be adjusted to a wide range of 50-1 800 mm². The velocity of droplets was greatly affected by the spray height. At a spray height of 50 mm， the velocity reached maximum value， with an average value as high as 14 m/s. Both the spray height and the auxiliary air flow rate significantly affect the viability of HaCaT cells. The rate of cell activation was 64.01±0.86% and 90.24±0.73% at a spray height of 50 mm and 100 mm， respectively （the cell activation rate was 92.98±3.21% in the negative control group）. Within 72 hours after spraying， the viability of HaCaT cells was consistent with that of unsprayed cells. The novel atomization device can be used for high-efficiency delivery of bio-ink， and the relevant research results also provide guidance for the optimal design of atomization parameters to achieve spraying of areas of different size and high cell-activation rate delivery.