• Journal of Radiation Research and Radiation Processing
  • Vol. 41, Issue 1, 010301 (2023)
Yizhe XUE, Zhaowen ZHANG, Ling GUO, Panpan LAI, Jiajin LIN, Tongzhou QIN, Yuntao JING, Xing WANG, Guiqiang ZHOU, and Guirong DING*
Author Affiliations
  • Department of Radiation Protection Medicine, School of Military Preventive Medicine, Air Force Medical University, Xi'an 710032, China
  • show less
    DOI: 10.11889/j.1000-3436.2022-0062 Cite this Article
    Yizhe XUE, Zhaowen ZHANG, Ling GUO, Panpan LAI, Jiajin LIN, Tongzhou QIN, Yuntao JING, Xing WANG, Guiqiang ZHOU, Guirong DING. Effects of 5G mobile phone radiation on sperm quality and sex hormone levels in mice[J]. Journal of Radiation Research and Radiation Processing, 2023, 41(1): 010301 Copy Citation Text show less
    Effects of continuous exposure to 4.9 GHz RFR for 21 days on the general health of mice: (a) body weight of mouse; (b) testis weight; (c) testis coefficient; data are expressed as Mean±SD, n=12
    Fig. 1. Effects of continuous exposure to 4.9 GHz RFR for 21 days on the general health of mice: (a) body weight of mouse; (b) testis weight; (c) testis coefficient; data are expressed as Mean±SD, n=12
    Effects of continuous exposure to 4.9 GHz RFR for 21 days on the morphological structure of seminiferous tubules in testis tissue of mice: (a) HE staining results of testis tissue, n=3; (b) diameter of seminiferous tubules; (c) thickness of seminiferous epithelium
    Fig. 2. Effects of continuous exposure to 4.9 GHz RFR for 21 days on the morphological structure of seminiferous tubules in testis tissue of mice: (a) HE staining results of testis tissue, n=3; (b) diameter of seminiferous tubules; (c) thickness of seminiferous epithelium
    Effects of continuous exposure to 4.9 GHz RFR for 21 days on epididymal morphology and sperm quality in a mice: (a) HE staining of epididymal tissue, n=3; (b) sperm count; (c) sperm abnormality rate
    Fig. 3. Effects of continuous exposure to 4.9 GHz RFR for 21 days on epididymal morphology and sperm quality in a mice: (a) HE staining of epididymal tissue, n=3; (b) sperm count; (c) sperm abnormality rate
    Effects of continuous exposure to 4.9 GHz RFR for 21 days on serum sex hormones in adult male C57 mice: (a) testosterone content; (b) FSH content; (c) LH content; (d) GnRH content, *pvs. Sham; **pvs. Sham; data are presented as Mean±SD, n=11~12
    Fig. 4. Effects of continuous exposure to 4.9 GHz RFR for 21 days on serum sex hormones in adult male C57 mice: (a) testosterone content; (b) FSH content; (c) LH content; (d) GnRH content, *p<0.05 vs. Sham; **p<0.01 vs. Sham; data are presented as Mean±SD, n=11~12
    Effects of continuous exposure to 4.9 GHz RFR for 21 days on the secretory function of sertoli cells in mice: (a) GDNF concentration; (b) SCF concentration; data are presented as Mean±SD, n=6
    Fig. 5. Effects of continuous exposure to 4.9 GHz RFR for 21 days on the secretory function of sertoli cells in mice: (a) GDNF concentration; (b) SCF concentration; data are presented as Mean±SD, n=6
    Effects of continuous exposure to 4.9 GHz RFR for 21 days on the secretion of related proteins from sertoli cells in mice: (a) representative results of Western blot; (b) relative expression of GDNF; (c) relative expression of SCF; data are presented as Mean±SD, n=3
    Fig. 6. Effects of continuous exposure to 4.9 GHz RFR for 21 days on the secretion of related proteins from sertoli cells in mice: (a) representative results of Western blot; (b) relative expression of GDNF; (c) relative expression of SCF; data are presented as Mean±SD, n=3
    Effects of continuous exposure to 4.9 GHz RFR for 21 days on blood-testis barrier tight junction-related proteins in mice: (a) representative bargraphs of Western blot; (b) ZO-1 gray value; (c) occludin gray value; data are expressed as Mean±SD, n=3
    Fig. 7. Effects of continuous exposure to 4.9 GHz RFR for 21 days on blood-testis barrier tight junction-related proteins in mice: (a) representative bargraphs of Western blot; (b) ZO-1 gray value; (c) occludin gray value; data are expressed as Mean±SD, n=3
    抗体名称 Antibody种属来源 Species公司 Company产地 Origin稀释比 Dilution ratio

    抗β-肌动蛋白

    Anti-β-actin

    小鼠单克隆抗体

    Mouse monoclonal Ab

    CMCTAG

    密尔沃基,美国

    Milwaukee, USA

    1∶5 000
    抗神经营养因子兔多克隆抗体Abcam剑桥,英国1∶400
    Anti-GDNFRabbit Polyclonal AbCambridge, England
    抗干细胞因子兔多克隆抗体SAB马里兰州,美国1∶300
    Anti-SCFRabbit Polyclonal AbMaryland, USA

    抗ZO-1

    Anti-ZO-1

    兔多克隆抗体

    Rabbit Polyclonal Ab

    Proteintech武汉1∶1 000
    Wuhan

    抗Occludin

    Anti-Occludin

    兔多克隆抗体

    Rabbit Polyclonal Ab

    Proteintech

    武汉

    Wuhan

    1∶2 000
    Table 1. Western blotting primary antibody details
    Yizhe XUE, Zhaowen ZHANG, Ling GUO, Panpan LAI, Jiajin LIN, Tongzhou QIN, Yuntao JING, Xing WANG, Guiqiang ZHOU, Guirong DING. Effects of 5G mobile phone radiation on sperm quality and sex hormone levels in mice[J]. Journal of Radiation Research and Radiation Processing, 2023, 41(1): 010301
    Download Citation