This paper proposed a readout integrated circuit (ROIC) with high uniformity and low-noise, by mitigating the fixed patter noise (FPN) of Infrared focal plane arrays (IFPA) to acquire high-quality infrared images. The row shared gain-controlling NMOS transistors are adopted in front-end circuit to reduce the pixel FPN. Furthermore, a novel correlated double sampling (CDS) structure is proposed to reduce the column FPN. Based on the simulation results, a 16×16 experimental chip has been manufactured adopting AMS 0.35 μm CMOS process. Extensive experiments have been implemented to verify the function and performance of the proposed readout circuit. The test results demonstrate the ROIC with the inherent advantages of low FPN and high uniformity, which makes it suitable for the application of high performance IFPA.