In order to study the stability of graphene transparent conductive film under light irradiation and the mechanism of its stability change, the electrical conductivity of graphene under different atmosphere, wavelength and power density of visible light irradiation were introduced. Results show that resistance of graphene film increase slowly under irradiation, and decrease after light off. The resistance of graphene film is most stable under nitrogen, and most unstable under vacuum. Under the irradiation of red light with the same power density, the relative resistance changes of samples under nitrogen, air and vacuum is 0.09%, 0.22% and 0.4% respectively. In the same environment, the shorter the wavelength, the higher the photon energy of the light source has the greater impact on the samples. In the atmosphere, with the same power density, the relative resistance of the sample changes 20% under blue light, compared to 3% for green and 0.22% for red.