• Chinese Journal of Lasers
  • Vol. 50, Issue 21, 2107106 (2023)
Yizhe Liu, Weisong Zhao, Yuzhen Liu, and Haoyu Li*
Author Affiliations
  • School of Instrumentation Science and Engineering, Harbin Institute of Technology, Harbin 150080, Heilongjiang, China
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    DOI: 10.3788/CJL230653 Cite this Article Set citation alerts
    Yizhe Liu, Weisong Zhao, Yuzhen Liu, Haoyu Li. Self-Adaptive Mixed-Emitter Single-Molecule Localization Algorithm[J]. Chinese Journal of Lasers, 2023, 50(21): 2107106 Copy Citation Text show less
    Reconstructed images of two 200 nm stripes apart generated by different algorithms, density of the strip is 1 μm-2 (above) and 51 μm-2 (below), respectively
    Fig. 1. Reconstructed images of two 200 nm stripes apart generated by different algorithms, density of the strip is 1 μm-2 (above) and 51 μm-2 (below), respectively
    Flowchart of SM algorithm, where the process of binary SNR generation, emitter identification and self-adaptation fitting algorithm are represented by yellow, blue and orange arrows, respectively
    Fig. 2. Flowchart of SM algorithm, where the process of binary SNR generation, emitter identification and self-adaptation fitting algorithm are represented by yellow, blue and orange arrows, respectively
    Simulation data and algorithm comparison on simulation data. (a) Simulated images with different densities; (b) comparison of metrics of different algorithms; (c) localization error of different algorithms; (d) simulated reference image (ground truth,GT); (e) sectional intensity profiles and local magnification of reconstructed super-resolution images by different algorithms
    Fig. 3. Simulation data and algorithm comparison on simulation data. (a) Simulated images with different densities; (b) comparison of metrics of different algorithms; (c) localization error of different algorithms; (d) simulated reference image (ground truth,GT); (e) sectional intensity profiles and local magnification of reconstructed super-resolution images by different algorithms
    Algorithm comparison on experiment data. (a) rFRC maps of super-resolution images obtained from different algorithms; (b)‒(c) local magnification of super resolution images; (d) sectional intensity profile corresponding to white lines in Fig. 4(b)
    Fig. 4. Algorithm comparison on experiment data. (a) rFRC maps of super-resolution images obtained from different algorithms; (b)‒(c) local magnification of super resolution images; (d) sectional intensity profile corresponding to white lines in Fig. 4(b)
    AlgorithmRMSESSIMPSNR
    SE1.18×1030.55921
    SM0.78×1030.69923.93
    ME1.16×1030.3421.15
    Table 1. Comparison of super-resolution images recovered from different algorithms with ground truth image
    AlgorithmMin /nmMean /nmMax /nm
    SE19.442.22551.39
    SM19.434.45100.82
    ME19.8544.14200.23
    Table 2. Quantitative resolution features of different algorithms given by rFRC
    AlgorithmMismatch errorCrosstalk errorAdvantage densityPhoto utilization efficiencyComputational complexitySpeed
    SENoneMuchLowLowSimpleHigh
    MEMuchLittleHighHighComplexLow
    SMNoneNoneFullHighSimpleMiddle
    Table 3. Comparison of SE, ME and SM algorithms
    Yizhe Liu, Weisong Zhao, Yuzhen Liu, Haoyu Li. Self-Adaptive Mixed-Emitter Single-Molecule Localization Algorithm[J]. Chinese Journal of Lasers, 2023, 50(21): 2107106
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