A mixed-language programming full automatic laser Z-scan measurement system based on LabVIEW and MATLAB is designed to express nonlinear optical features of samples. Full automatic control of experimental system measurement is realized using LabVIEW2012 development platform. MATLAB data analysis program is directly called through MATLAB Script node in LabVIEW environment so as to fit experimental data automatically and the nonlinear optical coefficient is obtained directly. Based on the calibration test of standard sample CS2, the measured third-order nonlinear refraction coefficient accords with the reported data, and the measurement process is rapid. Experimental results show that the complex manual operation is simplified by the system, automatic measurement of Z-scan system and integration of data processing are realized successfully. And the accuracy and efficiency of the measurement are improved.