Quercetin is a natural flavonoid compound that is used for the prevention and treatment of hypertension, hyperlipidemia, cardiovascular disease, cancer, etc. Therefore, quantitative detection of quercetin was particularly important in biochemistry and clinical medicine. A highly selective and sensitive detection methodfor quercetin with AIE (aggregation-induced luminescence phenomenon) fluorescent molecules was proposed, which identified the target molecule quercetin through coordination interaction with excited state electron transfer. The fluorescence of DSAZn in PBS buffer at pH 7.0 with adding five drug molecules (quercetin, icaritin, isorhamnetin, rutin and dopamine) was studied.The fluorescence emission spectra of 435~680 nm were scanned by a fluorescence spectrophotometer with an excitation wavelength of 415 nm. The ultraviolet absorption spectrum of 250~750 nm was scanned by ultraviolet spectrophotometer, which showed that the traditional Chinese medicine molecule quercetin could form a complex with the AIE fluorescent probe and thusstaticallyquenchedthe fluorescence of the AIE probe. Fluorescence detection showed that the quenching effect of five drug molecules on the fluorescent probe was significantly different. The binding constant of quercetin and DSAZn was 1.34×107 L·mol-1, which was an order of magnitude higher than that of the other four drug molecules, exhibitinga good selectivity for quercetin. The detection limit of quercetin was 3.07 nmol·L-1, which was lower than the value reported in many kinds of literature, exhibiting a high sensitivity for quercetin. The titrationcurve equation of quercetin to DSAZn based on the fluorescence titration spectrum was: y=0.013 4x-0.294 82. The linear range of quercetin concentration was 0～5 μmol·L-1 with a linear correlation coefficient of 0.994 3. Thus, a highly selective and sensitivedetection method for quercetin by AIE-type fluorescent molecules was constructed. This method was simply operated and repeatable, which provided a new research idea for detecting drugs with similar structures.