Cryosphere is one of the five most important spheres, which impacts the global climate system and has a close correlation with the sustainable development of the socio-economic system and the natural ecosystem. It serves as an important water source and solid water reservoir in cold and arid regions of China, the most developed country in terms of cryospheric in the middle and low latitude area. Widely distributed glacier, permafrost and snow cover not only have irreplaceable climatic effects, but also play a key role in guaranteeing economic development and ecosystem stability of the oasis in China's cold and arid regions. During the past decades, the studies in this field mainly focus on the adverse impacts of cryospheric; only a few are on cryospheric services and their profitability from socio-economic perspectives. There are even fewer studies on the comprehensive regionalization of cryospheric services. It becomes increasingly prominent to find the way to identify and quantify the structure of cryospheric services and their constituent elements, while we strengthen the scientific understanding of the spatial heterogeneity, complexity, and characteristics of cryospheric services. According to the development needs of the cryospheric science, this paper provides the research on comprehensive regionalization of cryospheric services and hopes to make necessary adjustments to the mutual promotion and coercion relationship of the cryospheric services. On one hand, it meets the major strategic needs of the Belt and Road Initiative, and considers the preparation for the Winter Olympics and the ecological progress; on the other hand, it contributes to mitigating risks and making reasonable use of cryospheric services. Firstly, a classification system is established for cryospheric services. Then, guided by the supply and demand equilibrium model, an importance evaluation of each cryosphere service is carried out, including supply-side importance evaluation, demand-side importance evaluation and the "developmental negative list" for amendment. Afterward, the location entropy algorithm is employed to determine the spatial distribution of the major cryosphere service under the guidance of service maximization model. Finally, the three-dimensional cube expansion method is applied into the comprehensive regionalization of cryospheric services, which divides the study area into multiple spatial levels, such as relatively independent, complete and organically linked units. To sum up, a comprehensive regionalization research system that integrates the natural supply and human demand and suits cryosphere characteristics is constructed. This paper aims at setting up a bridge between the supply of cryospheric services and the needs of social and economic development. It provides a reference for solving one of the key and urgent scientific issues regarding cryospheric science and the sustainable development of the man-earth areal system.