Metal-enhanced fluorescence (MEF) theory is a hot research topic in recent years. In this study, the fluorescence intensity and photon dynamic treatment (PDT) enhancement of gold nanobipyramids (Au NBPs) with different aspect ratios (3.1-6.5) to photosensitizer AlPcS is quantitively analyzed using spectral measurement, cell confocal imaging and flow cytometry method (FCM). The results of flourscence spectrum and confocal imaging show that Au NBPs with high aspect ratios can greatly enhance the fluorescence intensity of AlPcS, and the maximum enhancement factor is 6. Au NBPs with low aspect ratio can greatly reduce the fluorescence intensity of AlPcS, because its plasma resonance band is close to the fluorescence band of AlPcS. Hela cells (human epithelial cervical cancer cells) apoptosis measurements show that the join of Au NBPs, especially Au NBPs with high aspect ratios, improves drug-loading rate and singlet oxygen production of AlPcS. Then the survival rate of Hela cell is reduced and PDT effect of AlPcS is enhanced. These results provide a new avenue for the research of surface-enhancement fluorescence and expand the bioapplications of metal nanoparticles.