The variation in normal observers’ color perception and discrimination will occur with the aging effect. The authors carried out a psychophysical experiment with the method of binocular observational technique. Three observers at the age of 21, 32 and 58 were organized to match the 9 printed color samples on EIZO monitor with the repetition of 7 times. Groups of data for cross-media color matching with different age were established to investigate the response of photoreceptor cells. The results indicated that with the aging effect the chroma of the matched color will decrease obviously and the spectral response of the blue channel will decrease for blue and purple colors. The results validate the prediction of CIEPO06 model to some extent and provide some theoretical basis and experimental supports for the application of color reproduction and the color evaluation of the products related to age.