The multi-photon ionization spectrum of NO in the wavelength region of 575-680 nm is obtained with an optical parameter generator and amplifier (OPG/OPA) pumped by a picosecond Nd:YAG laser as radiation source. The banded structure of the spectrum indicates that NO molecule is ionized in resonant manner and the peaks of the spectrum are assigned to the transition of NO molecule from the ground electronic state to A2'Sigma' (v'=0,1,2,3), E2'Sigma' (v'=0,1,2), F2'Delta' (v'=0,1,2,3) and H2'Sigma' (v'=0,1,2) intermediate resonant ones. The molecule constants about NO (A2'Sigma', E2'Sigma', F2'Delta', H2'Sigma') states are calculated from the center wavelength of the spectrum. It is also found that owing to the special electron configuration of NO, this molecule does not follow the normal transition selection rule of the diatomic molecule during the multi-photon process.