Nurfatihah Che Abd Rashid, Nor Hafizah Ngajikin, Asrul Izam Azmi, Rashidah Arsat, Suhaila Isaak, Noran Azizan Cholan, Nur Ellina Azmi. Spectrophotometer with enhanced sensitivity for uric acid detection[J]. Chinese Optics Letters, 2019, 17(8): 081701
- Chinese Optics Letters
- Vol. 17, Issue 8, 081701 (2019)
Fig. 1. Configuration of spectrophotometer.
Fig. 2. Samples with different uric acid concentrations (a) before and (b) after stirring.
Fig. 3. Light beam at 10 mm distance from the input fiber. (a) Halogen beam. (b) Gray-scale percentage for halogen light. (c) LED beam (460 nm wavelength). (d) Gray-scale percentage for LED light.
Fig. 4. Spectrometer output spectra. (a) Halogen. (b) LED (460 nm wavelength).
Fig. 5. Light absorbance with variations of uric acid concentration at wavelengths of 460, 525, and 630 nm. (a) Halogen. (b) LED.
Fig. 6. Human urine sample in cuvette after mixing with NaOH solution.
Fig. 7. Absorbance analysis on human urine sample at 460 nm wavelength.
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Table 1. Comparison of Light Characteristics Between Halogen and LED Light Sources at 460 nm Wavelength
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Table 2. Comparison of Spectrophotometer Performance
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