AbstractIntroducing the top partner is a common way to cancel the largest quadratically divergent contribution to the Higgs mass induced by the top quark. In this work, we study single top partner production in the tZ channel at eγ collision in the littlest Higgs model with T-parity (LHT). Since it is well known that polarized beams can enhance the cross section, we analyze the signal via polarized electron beams, and photon beams. we have selected two decay modes for comparison, based on the leptonic or the hadronic decays of the W and Z from the top partner. We then construct a detailed detector simulation, and choose a set of cuts to enhance signal significance. For mode A(B), the capacity for exclusion in this process at s = 3 TeV is comparable to the current experimental limits with L = 1000 (500) fb−1. If the integrated luminosity can be increased to 3000 fb−1, the top partner mass mT+ can be excluded up to 1350 (1440) GeV at 2&sgr; level. We also considered the initial state radiation effect, and find that this effect reduces the excluding ability of the eγ collision on the the top partner mass by approximately 10 GeV. Moreover, the ability to exclude the LHT parameter space at eγ collision complements the existing research.