• Optics and Precision Engineering
  • Vol. 32, Issue 16, 2455 (2024)
Meichen LIU1,2, Qingyu MENG1,*, Hongwen ZHANG1, Kejun WANG1..., Yunhui LI1, Siqi WANG1,2 and Jiangnan WANG1,2|Show fewer author(s)
Author Affiliations
  • 1Changchun Institute of Optics,Fine Mechanics and Physics,Chinese Academy of Sciences, Changchun30033,China
  • 2University of Chinese Academy of Sciences,Beijing100049,China
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    DOI: 10.37188/OPE.20243216.2455 Cite this Article
    Meichen LIU, Qingyu MENG, Hongwen ZHANG, Kejun WANG, Yunhui LI, Siqi WANG, Jiangnan WANG. Design of gene sequencing fluorescence microscopic system with dual-band LED illumination[J]. Optics and Precision Engineering, 2024, 32(16): 2455 Copy Citation Text show less
    Optical path of dual-band gene sequencing fluorescence microscopic imaging system
    Fig. 1. Optical path of dual-band gene sequencing fluorescence microscopic imaging system
    Relation spectral power distribution of R-LED and G-LED and transmission of filter at different wavelengths
    Fig. 2. Relation spectral power distribution of R-LED and G-LED and transmission of filter at different wavelengths
    Layout of the illuminating optical system
    Fig. 3. Layout of the illuminating optical system
    Schematic of the illumination system simulation
    Fig. 4. Schematic of the illumination system simulation
    Layout of the imaging optical system
    Fig. 5. Layout of the imaging optical system
    Structure of radiator
    Fig. 6. Structure of radiator
    Structure of the optical filters
    Fig. 7. Structure of the optical filters
    Structure of radiator
    Fig. 8. Structure of radiator
    Complete machine system
    Fig. 9. Complete machine system
    Imaging test
    Fig. 10. Imaging test
    Result of testing
    Fig. 11. Result of testing
    Schematic diagram of sampling
    Fig. 12. Schematic diagram of sampling
    ParameterSpecifications
    Resolution/pixel5 472×3 648
    Pixel size/μm22.4×2.4
    Excitation wavelength/nm525, 620
    Fluorescence wavelength/nm570 ,680
    Imaging range/mm1.31×0.88
    Systemic resolution/μm≤2
    Table 1. Specification of the optical system
    Meichen LIU, Qingyu MENG, Hongwen ZHANG, Kejun WANG, Yunhui LI, Siqi WANG, Jiangnan WANG. Design of gene sequencing fluorescence microscopic system with dual-band LED illumination[J]. Optics and Precision Engineering, 2024, 32(16): 2455
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