In this paper, imaging characteristics with different light sources based on volume holographic imaging system are researched. The volume holographic lens as imaging lens is recorded in the 2-mm-thick LiNbO3FeCu crystal at wavelength of 532 nm. The imaging characteristics of the imaging system are researched at the reading wavelength of 532 nm and 640 nm. The results show that the depth resolution of volume holographic imaging system is different when different reading wavelengthes are used. Axial selectivity is more sensitive when the red light is used as the reading light. The imaging features of monochromatic light source and broadband light source are compared. Experimental results show that using broadband light source can obtained more transveres information of the object.