An improved genetic algorithm was used to implement an automated wavelength selection procedure for use in building multivariate calibration models based on partial least squares regression (PLS).The region selecting by genetic algorithms (R-SGA) was applied in building calibration model of soluble solid content (SSC) of Western pear,and the numbers of latent variables used to build calibration model were further reduced.The Fourier transform near infrared reflectance (FT-NIR) spectra were processed by GA after MSC or SNV,and four PLS calibration models were built by using the optimal combinations of these sub-regions.Meanwhile,the full region selecting PLS (Fr-PLS) models were developed.The R-SGA models variables were 434,496,310 and 496,for Early Red Comice,Wujiuxiang,Cascade and Kang Buddha,respectively.Despite the complexity of the spectral data,the R-SGA procedure was found to perform well (RMSEP=0.428,0.567 for Early Red Comice and Kang Buddha,respectively),leading to calibration models that significantly outperform those based on full-spectrum analyses (RMSEP=0.518,0.633).The prediction precision of GA-PLS models was similar to that of Fr-PLS for Wujiuxiang and Cascade,with RMSEP of 0.696/0.694 and 0.425/0.421 respectively.This work proved that the R-SGA could find optimal values for several disparate variables associated with the calibration model and that the PLS procedure could be integrated into the objective function driving the optimization.