This study uses the MATLAB App Designer software based on the principles of point kernel integration and the Monte Carlo method to design an interface program for the cobalt source layout effect of gamma irradiation equipment. The interface program can simulate the manual and automatic layout calculation processes of cobalt sources, display real-time changes in the cobalt source position of the operation module and the entire source intensity effect map, and rapidly calculate the surface dose distribution of the source rack and the spatial dose distribution map of the product. Under the expected parameter conditions, the interface program can automatically calculate and select the optimal source discharge scheme by importing information such as the cobalt source activity and position. The layout scheme exported by the interface program can be used to execute the GEANT4 Monte Carlo program directly, thereby verifying the accuracy of the layout scheme. By comparing the concentration and dispersion of different cobalt source activities, the results show that the interface program agrees well with the GEANT4 calculations. Adding cobalt sources or adjusting the layout plan is important for selecting the optimal plan, improving the utilization rate of cobalt sources, and increasing the production capacity of irradiation equipment.