A two-dimensional (2D) holographic polymer-dispersed liquid crystal (H-PDLC) variable line-space grating is fabricated by interference between cylindrical wave and plane wave. In order to separate polymer from liquid crystal more evidently, we use a 532 nm laser with exposure intensity of 16 mW/cm2 in experiment, and two exposure time is 2 s and 60 s, respectively. We use double exposure and rotate the sample 60° to form 2D hexagonal lattice H-PDLC variable line-space grating. The theoretical periodic variation rang of grating, diffraction properties, electronic control characteristics and analysis of parameters affecting the periodic change of grating are studied, respectively. The results show that, in the circular range with a radius of 6 mm, the periodic variation range of the grating is 1.804-2.281 μm, which is basically consistent with the theoretical calculation. The first-order diffraction efficiency is 18.3% without external voltage, and the zero-order diffraction efficiency increases from 15.6% to 73% with an applied voltage of 90 V. Due to variable period and electronic control characteristics, this grating has potential application in diffraction optics, such as the research of tunable multi-wavelength organic laser for dye-doped 2D H-PDLC grating.