This paper presents the design,simulation and fabrication of edge-lit Light-Guide Plates(LGPs) using laser micro-machining technique for Liquid crystal display backlights. The optical structure of the LGP is established in an optical software,and simulated by tracing rays using Monte Carlo method. The designed microstructures with gradually changing desity are proved to offer output homogeneous illuminance. Based on the simulation results,Laser Micro-machining Technique(LMT) fabrication is performed for verification. The measured size of the fabricated microstructure is approximately 30.14 μm under 100× magnification,which is close to the prescribed size of 30 μm. The 3D surface morphology of the fabricated microstructure is also available. Ten groups of edge-lit LED display backlight prototypes equipped with the LGPs fabricated by LMT and Screen Printing Technique(SPT) are developed and analyzed. Comparison results show that LGPs fabricated by LMT can provide better optical uniformity (6.2% higher) and lower color difference (0.002 7 lower) than conventional method (SPT). Besides the advantages of faster and simpler,LMT is entirely environmentally-friendly during the machining process. With the characteristics of good performance,automatic production and thinning tendency,it is reasonably believed that LMT will have bright prospect in the near future,and may be a promising alternative method for SPT in display applications.