Through the influence of the He-Ne laser irradiation of 5 mW/mm2 He-Ne, 10.08 kJ/(m2·d) UV-B and the combination of the two on the seeding of winter wheat "Linyou 2018", the modification of apoptosis on the roots of seedling in different treatment days and each group are analyzed. The research results indicate that, the features of apoptosis are found in the roots of wheat seedling after enhanced UV-B irradiation, such as nuclear assembled, apoptopic bodies. Apoptosis in different treatment groups by flow cytometry are quantitative analyzed. The results also show that the number of apoptosis are increased after enhanced UV-B irradiation and the differences are obvious in the 5 th day. With the management of He-Ne laser after UV-B radiation, the number of apoptosis is less than that of the UV-B treatment group and decreases obviously. Therefore, the apoptosis of roots of wheat seedling induced by enhanced UV-B radiation can be remited partly by He-Ne laser irradiation.