Effects of 5-aminolevulinic acid-mediated photodynamic therapy (ALA-PDT) with low energy on intracranial angiogenesis and glioma growth are evaluated. Twenty seven nude mice are received low energy of ALA-PDT (ALA: 300 mg/kg, dose: 10 J/cm2) on the right side of the cerebral cortex. After 1、5、10 days, angiogenesis is evaluated by 2 and 3 dimensional vessel images, and expressions of VEGF and hypoxia-inducible factor-1α (HIF-1α) by western blot. Other 12 nude mice are divided into control and ALA-PDT (10 J/cm2) pretreated groups. Ten days after pretreatment, mice are implanted intracerebral U87 glioma; and tumor volume is calculated by H.E staining after 21 days. Results show that the vessels in the contralateral brain are as control. Both 2 and 3 dimensional vessel images shown that there is no difference in the microvessels morphology on the first and fifth days after ALA-PDT compared with control. However, there is angiogenesis on the tenth days showing as decreased length, increased diameter and the number of branch points. VEGF expression significantly increased on fifth day and becomes higher on the tenth days after ALA-PDT, although there is no change on the first day; while HIF-1α expressions increased on the first day and become higher and higher. ALA-PDT with low energy can induce VEGF expression and intracerebral angiogenesis mediated by HIF-1α, and this effect promotes glioma growth in brain.