A realization of 3D-mouse is proposed.By the principle of stereo vision,the method based on autostereoscopic Display can make the common mouse point to any location at different depths in three-dimensional space which can be perceived by the viewers.And,a method of calculating the real position’s depth is also proposed and is verified experimentally.Theoretical analysis and experiment results show that the method of the realizing 3D-mouse and calculating the real position’s depth can make the autostereoscopic display be introduced into the digital photogrammetric system.