The bonding of glass and titanium alloy is widely applied in roof, curtain wall, windows due to its excellent performance such as high strength, light weight and corrosion resistance. An experimental study of welding of dissimilar materials between transparent architectural glass and titanium alloy (TC4) using a pulsed mode 300 W Nd:YAG laser is reported. The process is achieved when the joint is irradiated from the glass side. The test of breaking strength is conducted by universal tensile test machine. The fracture morphology on glass side and microstructure of weldment are analyzed using scanning electron microscopy. The influence of process parameters (power, pulse duration, pulse repetition rate, defocus distance) on weld seam formability, breaking strength and fracture morphology are studied. The results indicate that a good glass-to-TC4 joint can be obtained using millisecond pulsed laser. The maximum breaking strength of the weldment is 140.76 N. The mixed structure of glass and TC4 at the interface plays an important role in connecting the two materials, and adhesion particles play a subsidiary role.