In this paper, a new method for the rapid, economical and convenient detection of cyclic adenosine monophosphate (cAMP) in jujube is proposed and verified. Based on near-infrared (NIR) fiber spectroscopy combined with stoichio-metric analysis, the cAMP content in red jujube can be quickly detected. 68 red jujube samples were used for the NIR spectroscopy data acquisition and the corresponding chemical values were determined. The sample set was adjusted based on the joint XY distance (SPXY) to select the correction sample set. After different preprocessing on the spectra, the partial least squares (PLS) method was used to establish the model, and the smoothed and normalized PLS model result was obtained better. The model's correction correlation coefficient (Rc), correction set mean square error (RMSEC), prediction correlation coefficient (Rp), and prediction and mean square error (RMSEP) are 0.951 5, 25.793 7, 0.910 8 and 28.228 0, respectively. The results show that NIR combined with specific chemometric methods can achieve rapid de-tection of cAMP in red jujube.