The gold hollow-core semi-shell nanofilms with different thicknesses were fabricated by depositing gold onto a cleaned glass support covered with a close-packed monolayer of 200 nm diameter polystyrene spheres and subsequently by the removal of the PS spheres. The thicknesses of gold nanofilms can be controlled by the deposition time, the morphologies and optical properties of gold films were obtained by field emission scanning electron microscopy and home-built spectrometer respectively, and the relationship between morphologies and tunable plasmonic properties was also discussed. Then, using 4-aminothiophenol (4-ATP) as the probe molecule, the surface enhanced Raman scattering signals of 4-ATP adsorbed on the films after immersing the nanofilms into the 4-ATP solution were measured by Raman spectrometer with the excitation wavelength of 785 nm. The results show that the localized surface plasmon resonance wavelength of gold nanofilm red shifts as the film thickness increases, and the resonance wavelength can be tuned over a wide range from visible band to infra-red band; moreover, when the resonance wavelength of nanofilms is close to the incident excitation wavelength, the surface enhanced Raman scattering signals can be obtained obviously, indicating that the fabricated nanofilms can be used as the potential surface enhanced Raman substrates. The reasons of the shift of the spectral peak and SERS intensity of different nanofilms were also discussed.