The coherent superposition of incoming linearly polarized light and radially polarized light can generate the three-dimensional (3D) polarization optical field at the focal spot of a high NA microscopic objective. In addition, arbitrary 3D polarization orientation of focusing optical field can be obtained by adjusting the intensity ratio between two incoming beams and rotating the polarization orientation of the incoming linearly polarized light. Based on the vectorial diffraction theory, a simulation model was developed to theoretically verify the feasibility of the proposed control method of 3D polarization, and to evaluate the 3D polarization optical field generated from this control method. After experimental test on the basis of theoretical analysis and building the practical optical setup, the feasibility of the method was confirmed. It was also disclosed that this method and the corresponding optical path is simpler and easier to implement compared with other existing 3D polarization control methods.