Components like SiO2, Al2O3, Fe2O3, CaO, MgO and Cr2O3 dominate the performance of chromium-containing amorphous refractory. That’s still a hard task for product evaluation and quality control for amorphous refractory due to various component content, less available standard product and difficulty in fusion bead preparation. In the present study, series calibration standards were made from reagents and marked standards depend upon the content of components in real samples. The fusion bead was prepared with lithium tetraborate-lithium metaborate (67∶33 by weight) as mixed flux and ammonium iodide as release agent, which was added during fusion process. The calibration curve derivated from the relationship between light intensity and components concentration and calibrated by theoretical α coefficient. The results of measurement were consistent with standard by this newly developed method. And good precision was also achieved in determination of real amorphous samples. It is easy in operation with less time spent, and practical in application.