A support structure with an adhesive truss was designed for a space camera with off-axis multi-mirror systems. The struts of this truss were manufactured with T700 made of carbon fiber composites, fittings were manufactured with titanium alloys, the adhesive was J133 epoxy resin and casting titanium fittings were bonded on both ends of the truss struts to provide interfaces for other subassemblies of the camera. The methods and steps for assist alignment equipment and gluing the subassemblies were described particularly. Firstly, preliminary assembly and alignment were done to ensure the precise locations of struts and fittings using the assistance equipment. Then, the formal alignment was performed with the help of locating pins, and the struts and fittings were restored in the correct initial positions and bonded with adhesives. Finally, the structure was solidified at room temperature for 5-7 days, and the assembly and alignment were finished after dismantling the assistance equipment and pins.The results of finite element analysis and vibration test indicate that the first-order natural frequencies of the truss and the lens supported by the truss are more than 90 Hz and 70Hz, respectively. Interfaces of mirror subassemblies were measured before and after vibration tests, and the results show that the flatness has been reached to 0.15 mm and the maximum relative error of interface flatness is 5.3%. The conclusion is that the stiffness and dimensional stability of the truss well meet the design requirements.