Monodisperse Fe3O4@SiO2@CdTe magnetic and luminescent double functional composite microsphere were successfully prepared by amide condensation reaction with Fe3O4 magnetic nanoparticles coated by SiO2 with －NH2, which were got by hydrolyzing with (3-ammonium propyl) triethoxy silane and CdTe quantum dots modified by thioglycolic acid. Fe3O4 magnetic nanoparticles were received by hydrothermal synthesis method and stber method. The structures and properties of the composite microsphere were characterized by transmission electron microscopy, X-ray diffraction, fluorescence spectrophotometer and vibrating sample magnetometer. The results demonstrated that Fe3O4@SiO2@CdTe magnetic and luminescent composite microspheres have good monodispersity, the average particles size is 470 nm and the saturation magnetization is 37.9 emu/g. The submicron magnetic and luminescent composite particles have well superparamagnetism and high efficiency fluorescent light.