In order to fabricate vitreous microfluidic components with round cross sections and microchannel networks with nude structure,a hot rheologic deformation puller for fabricating vitreous three-way microchannel was designed.In the forming process of the three-way microchannel,the tip of the bended glass capillary with the shape of "V" was softened by heating and then was pulled into three-way microchannel in cooling process.The reservoirs connecting the microchannels with conical pipes were also formed during the pulling process.The three-way microchannels with both equal or unequal inner diameters could be made by the puller.The temperature in the heating field,the inner diameter of microchannel and the distribution coefficient of vitreous material could be adjusted by heating time,pulling distance and micro-feed mechanism,respectively.A three-way microchannel with inner diameter of 67,32 and 20 μm was fabricated,its three ways were connected smoothly in the joint.For surface tension forming,the three-way microchannel shows a higher quality surface.Finally,a microchannel network was assembled with the one-dimension microchannels and the three-way microchannels successfully.