• Journal of Innovative Optical Health Sciences
  • Vol. 7, Issue 1, 1350054 (2014)
LIXIN LIU1、*, JIA QIAN2, YAHUI LI1, XIAO PENG3, and JUN YIN3
Author Affiliations
  • 1School of Physics and Optoelectronic Engineering Xidian University, Xi'an 710071, P. R. China
  • 2Xi'an Institute of Optics and Precision Mechanics Chinese Academy of Sciences, Xi'an 710119, P. R. China
  • 3College of Optoelectronic Engineering Shenzhen University Shenzhen 518060, P. R. China
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    DOI: 10.1142/s1793545813500545 Cite this Article
    LIXIN LIU, JIA QIAN, YAHUI LI, XIAO PENG, JUN YIN. MONTE CARLO SIMULATION OF MULTIFOCAL STOCHASTIC SCANNING SYSTEM[J]. Journal of Innovative Optical Health Sciences, 2014, 7(1): 1350054 Copy Citation Text show less

    Abstract

    Multifocal multiphoton microscopy (MMM) has greatly improved the utilization of excitation light and imaging speed due to parallel multiphoton excitation of the samples and simultaneous detection of the signals, which allows it to perform three-dimensional fast fluorescence imaging. Stochastic scanning can provide continuous, uniform and high-speed excitation of the sample, which makes it a suitable scanning scheme for MMM. In this paper, the graphical programming language — LabVIEW is used to achieve stochastic scanning of the two-dimensional galvo scanners by using white noise signals to control the x and y mirrors independently. Moreover, the stochastic scanning process is simulated by using Monte Carlo method. Our results show that MMM can avoid oversampling or subsampling in the scanning area and meet the requirements of uniform sampling by stochastically scanning the individual units of the N × N foci array. Therefore, continuous and uniform scanning in the whole field of view is implemented.
    LIXIN LIU, JIA QIAN, YAHUI LI, XIAO PENG, JUN YIN. MONTE CARLO SIMULATION OF MULTIFOCAL STOCHASTIC SCANNING SYSTEM[J]. Journal of Innovative Optical Health Sciences, 2014, 7(1): 1350054
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