To ascertain the material, making technique, and possible sources of raw materials of some purple gemstone bead ornaments unearthed from the tomb dated to Han Dynasty in Jiuzhiling, Hepu, Guangxi, eight beads were analyzed by X-ray fluorescence spectroscopy (XRF), Raman spectroscopy and Digital microscope system. The results of XRF and Raman spectroscopy showed that these beads were made of pyralspite garnet group endmember species. They have similar chemical compositions to that of almandine-garnets, except one sample attributed to almandine-pyrope garnet. We discussed the processing techniques and the possible sources of the garnet beads based on the mark evidence obtained by Digital microscope system, and the recent results of archaeological and scientific researches. The rotary grinding-stone technique and the spherical polishing-leather bag technique were used for grinding and polishing these beads, and a double-diamond drills was used to drill the beads from both sides. It can be concluded that the batch of garnet gemstone beads were imported to Hepu from South Asia by way of Southeast Asia or from South Asia along maritime Silk Road. The beads analyzed in this paper are important evidences of the Maritime Silk Road trade in Han Dynasty. It is significant for the studies of the history and cultural exchange on the maritime Silk Road.