Two beams of pumping up-conversion three-dimensional (3D) volumetric display which is based on a ZBLANPr, Yb fluoride glass are investigated. The lasers employed are infrared semiconductor laser with wavelength 960 nm and tunable Ti gem laser with wavelength of 820 nm, respectively. The two frequency up-conversion luminescence of Pr3+ ion is investigated. The relation between up-conversion fluorescence intensity, pumping lasers intensity, and ion doped concentration is analyzed, and the experimental conditions to realize the clear two frequency up-conversion 3D display are therefore obtained. ZBLAN glass is selected as two-frequency up-conversion 3D volumetric display foundation material for it has perfect phonon spectrum whose maximum phonon frequency is 580 cm-1; up-conversion fluorescence intensity increases linearly with augment of two beams of pumping lasers intensity when lasers intensity are very weak; optimal doped concentration of Pr3+ and Yb3+ ions are 0.5% and 1.5% mole fraction, respectively.