In the present study, we evaluate the relative content of chlorophyll and spectral reflectance variations in the visible light under different intensity of UVB (L-UVB, CK and UVB) of three typical evergreen broadleaf plants in China subtropical area. In different simulated UVB condition, the experiment shows that different tree species have different UVB sensitivity, and chlorophyll content varies greatly with species, and the chlorophyll relative content with the filter UVB was significantly higher than with enhanced UVB. In the spectral reflectance of the visible part, it is generally higher with enhanced UVB’s treatment than with L-UVB treatment; and any treatments present adaptation, species under different stress. After roles of the different UVB intensity, for each tree species the visible part of the spectral reflectance shows difference between green and red mainly. The study results show that the subtropical evergreen broad-leaved species has a strong sensitivity to the UVB, and UVB response of different tree species varies greatly.