Diagnosis of Breast Cancer Using Gaussian Function to Fit Autofluorescence Spectrum

Wenjing Chen; Zhaohang Jiao; Dongli Qi; Longhai Shen; Nuo Xu; Dongfei Xie; Yifei Li; Jiahua You; Qi Li; Yu Feng

doi:10.3788/CJL202249.2007106

Journals >Chinese Journal of Lasers >Volume 49 >Issue 20 >Page 2007106 > Article

Chinese Journal of Lasers
Vol. 49, Issue 20, 2007106 (2022)

Diagnosis of Breast Cancer Using Gaussian Function to Fit Autofluorescence Spectrum

Wenjing Chen, Zhaohang Jiao, Dongli Qi, Longhai Shen, Nuo Xu, Dongfei Xie, Yifei Li, Jiahua You, Qi Li, and Yu Feng^*

Author Affiliations

College of Science, Shenyang Ligong University, Shenyang 110158, Liaoning, China

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DOI: 10.3788/CJL202249.2007106 Cite this Article Set citation alerts

Wenjing Chen, Zhaohang Jiao, Dongli Qi, Longhai Shen, Nuo Xu, Dongfei Xie, Yifei Li, Jiahua You, Qi Li, Yu Feng. Diagnosis of Breast Cancer Using Gaussian Function to Fit Autofluorescence Spectrum[J]. Chinese Journal of Lasers, 2022, 49(20): 2007106 Copy Citation Text

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Fig. 1. Schematic of experimental device

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Fig. 2. Fluorescence spectra of slide and stain. (a) Fluorescence spectra of slide; (b) fluorescence spectra of stain

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Fig. 3. Normalized mean fluorescence spectra of breast tissues

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Fig. 4. Normalized mean fluorescence spectrograms fitted by Gaussian function. (a) Normal breast tissue by SP staining; (b) unstained cancerous breast tissue; (c) cancerous breast tissue by HE staining; (d) cancerous breast tissue by SP staining

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Name	Instrument information
Laser	Wavelength: 405 nm; power: 150 mW
Dichroic mirror	Critical wavelength: 410 nm; transmission wavelength: >419 nm; transmittance: >80%; reflection wavelength: 360－405 nm; reflectance: >99%
Fiber probe	Diameter: 400 m; wavelength range: 300－800 nm
Fiber spectrometer	Spectral range: 200－1100 nm; scanning speed: 4500 scan·s^－1: resolution: 0.8 nm

Table 1. Experimental instrument information table

Sample	A₁	A₂	A₃	λ₁	λ₂	λ₃	ω₁	ω₂	ω₃	R²
SP(normal)	55.73	37.59	10.61	491.39	516.76	631.68	64.06	107.37	89.16	0.998
Unstained(cancer)	19.48	72.23	31.49	492.74	520.77	630.59	39.41	82.59	147.65	0.987
HE(cancer)	22.94	70.61	23.81	492.35	520.89	631.24	41.38	135.55	147.13	0.992
SP(cancer)	24.47	38.57	27.86	495.14	517.25	640.84	64.34	45.05	116.20	0.996

Table 2. Characteristic parameter information of Gaussian fitting peak

Breast tissue	A₅₁₇/A₄₉₂	A₆₃₅/A₄₉₂
Normal tissue with SP staining	0.6745	0.1904
Unstained cancerous tissue	3.7079	1.6105
Cancerous tissue with HE staining	3.0780	1.0379
Cancerous tissue with SP staining	1.5762	1.1385

Table 3. Peak area ratios of A₅₁₇/A₄₉₂ and A₆₃₅/A₄₉₂ of normal and cancerous breast tissues

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