Advances in optical coating technology over the past decade have made it possible to produce high-performance Raman spectroscopy filters with better reliability and at lower costs. The performance and characteristics of three typical Raman filters and an ultraviolet resonance Raman filter are introduced. Some applications of surface-enhanced Raman scattering (SERS) biosensors for the detection and identi-fication of tissues, cells, proteins, nucleic acids, drugs, and chemical pathogens are reviewed.